Perianal and perineal rhabdomyosarcomas: a retrospective multicenter study of 35 cases
BMC Surgery volume 21, Article number: 66 (2021)
Perianal/perineal rhabdomyosarcomas (PRMS) are easily misdiagnosed soft tissue tumours with a poor prognosis. This study was designed to analyze the clinical, diagnostic, pathological and prognostic features of PRMS, and to explore currently available therapeutic modalities.
Clinical data of PRMS patients admitted to the Sixth Affiliated Hospital and the Cancer Center of Sun Yat-sen University and from related Chinese literature published from 1987 to 2018 were collected and analyzed. The Chi-square test was used to evaluate the differences between each group. The Kaplan–Meier methods were applied to estimate and compare survival rates.
A total of 35 patients were included in this study; 20 identified within related Chinese literatures and 15 from our center admitted during the period of 1997–2019. Out of these cases, 34 presented with perianal masses and the remaining one manifested as an inguinal mass. Moreover, 20 patients complained of pain and 16 of them were misdiagnosed as perianal abscesses, in which the presence of pain contributed to the misdiagnosis (p < 0.05). The average time interval between symptom onset and pathological diagnosis was 3.1 months. Next, 13 cases were classified into IRS group III/IV and 20 cases into stages 3/4. Additionally, 14 and 9 cases received the pathological diagnoses of embryonal rhabdomyosarcoma and alveolar rhabdomyosarcoma, respectively. Regarding the patients’ survival rates, five patients survived for more than 2 years, and three of them survived for more than 5 years. The overall 2 years and 5 years survival rates were 32% and 24%, respectively. The symptom of pain and misdiagnosis both contributed to the poor prognosis in these patients (p < 0.05). MRI showed that the PRMS were closely related to external anal sphincter in 10 cases.
PRMS are easily misdiagnosed lesions, which often leads to an unfavourable outcome in affected patients. Patients with painful perianal masses should be evaluated to exclude PRMS. MRI revealed that PRMS are closely related to the external anal sphincter. Multidisciplinary management is recommended in the treatment of PRMS.
Rhabdomyosarcomas (RMS) are common soft tissue malignancies, but primary perianal and perineal rhabdomyosarcomas (PRMS) are extremely rare, accounting for only 2% of all RMS . PRMS are routinely misdiagnosed and mistreated, associated with a relatively high mortality rate. In this study, 35 cases were reviewed to clarify the clinical manifestations, imaging findings, pathologic and prognostic features of PRMS, as well as to explore the optimal therapeutic regimen for this condition.
Fifteen PRMS patients treated and followed up at the Sixth Affiliated Hospital (n = 6) and the Cancer Center (n = 9) of Sun Yat-sen University were reviewed in this study. The perianal region consists of the area posterior or lateral to the anus. The perineal region is clinically defined as the area between the anus and the scrotum or labia magna. Patients with gluteal or deep pelvic primaries involving the perineum or of urogenital origin were excluded . Patients’ baseline information was gathered and analyzed, including their gender, age at first diagnosis, symptoms, preoperative tumor size, lesion pathological classification, lymph node involvement, metastasis and treatment received. Postoperative staging and grouping were in accordance with the IRS (Intergroup Rhabdomyosarcoma Study) standard . A database of medical journals published in Chinese between 1987 and September 2018 was examined and 20 patients were identified [4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19]. Finally, a total of 35 patients were included in this study. This study obtained approval from the Ethics Committee of the Sixth Affiliated Hospital.
The Spss 26.0 software package was used for statistical analysis. The Chi-square test was used to assess the differences between each group. Kaplan–Meier methods were used to assess the Overall Survival (OS), which was calculated as the time from pathological diagnosis to the point of death or the last follow-up record. Survival curves among different patient subgroups were compared with the Mantel log-rank test. The threshold for statistical significance was set at P < 0.05.
Table 1 summarizes the data of the patients treated at our center, meanwhile the clinical characteristics of all the 35 patients were listed in Table 2. The study group comprised 10 males and 25 females within an age range of 1–56 years old. The most common presenting symptom was perianal/perineal mass, only one patient initially complained of an inguinal mass. Other symptoms including pain, hematochezia, difficult defecation and fecal incontinence (3%) were noted. Fifteen patients had regional lymphatic metastases and 5 patients had distant metastases at the time of diagnosis. The IRS classification was group I in 1, group II in 11, group III in 8 and group IV in 5 cases. The pre-treatment staging was stage-2 in 6, stage-3 in 15 and stage-4 in 5 cases.
Fifteen patients treated at our center received imaging evaluation. Four patients were examined by color Doppler ultrasound, which reviewed hypoechoic or mix-echoic masses with unclear boundaries and irregular shapes. Spot blood flow signals were detected by CDFI in 3 cases and 1 case had abundant blood flow signals around the mass. These patients were then subjected to ultrasound-guided biopsy and pathology confirmed the diagnosis of RMS. CT/MRI scans were performed for all the 15 patients, which delineated an average maximum tumour diameter of 6.8 cm (1.8–12.2 cm). Tumour compression of the internal anal sphincter and rectal mucosa was observed in 5 cases and 3 of them had urogenital involvement. MRI was conducted in 10 patients and demonstrated equal signal on T1WI (n = 10) and high (n = 5) or mixed (n = 5) signals on T2WI within tumours. Furthermore, external anal sphincter (EAS) involvement was observed in all of the 15 cases (Fig. 1), including 3 having puborectalis involvement and 3 with levator ani involvement (Fig. 2). Regional lymph node metastases were found in 12 patients. Moreover, 5 patients had distant metastases at distant sites including bones, lungs, pancreas, breasts and the pelvic cavity. Clinical stages were determined based on imaging evaluation as follows: 5 cases in stage-2, 6 in stage-3 and 4 in stage-4.
Six of 15 and 9 cases in our group were histologically confirmed as alveolar RMS (Fig. 3) and embryonal (Fig. 4), respectively. Myogenin was expressed in all cases. Desmin and vimentin were positive in 5 of the 5 cases. MyoD1 was positive in 3 out of 4 cases. Of all the 35 patients, 14 were classified as embryonal RMS, 9 were labeled as alveolar RMS and 1 was identified as pleomorphic RMS. Furthermore, 11 cases had no histological reports. A total of 20 cases had immunohistochemical records reporting the following: Desmin (+) (20/20), vimentin (+) (14/14), myogenin (+) (15/15), MyoD1 (+) (9/10) and myoglobin (+) (9/9).
Treatments and outcomes
Ten patients received surgical treatment at our center. Primary surgery was performed for 3 patients (R0 = 2, Rx = 1) and secondary surgery after chemotherapy was performed for 6 patients (R0 = 3, R1: n = 1; Rx: n = 2). Among these patients, 4 received an abdominoperineal resection (APR) and 5 received a local radical tumor resection (LRR). One patient only underwent an inguinal lymphadenectomy only. All 15 patients in our center received a chemotherapeutic regimens such as MAID (Mesna, adriamycin, isocyclic amide, dacarbazine), CYVADIC (Cyclophosphamide, vincristine, adriamycin, dacarbazine), CEVAIE (Carboplatin, epirubicin, vincristine, dactinomycin, ifosfamide, etoposide), FOLFOX (Fluorouracil, oxaliplatin, calcium folinate) and TP (Cisplatin, paclitaxel) regimens. Three patients received radiotherapy (RT) postoperatively and one preoperatively. The median survival time was 14 months (ranging from 8 to 120 months). Nine patients died of disease progression after diagnosis (8–25 months).
Taking into account the information retrieved from the Chinese database, 18 out of the 35 patients underwent surgical treatment (APR: n = 7; LRR: n = 11) and 9 of them received radiotherapy or chemoradiotion therapy (CRT) alone. The treatment modality was unknown in 8 cases. Fifteen patients were misdiagnosed as perianal abscesses, 14 of them underwent an abscessotomy and 1 was treated with intravenous antibiotics only. One patient was misdiagnosed as anal fistula with subsequent fistula resection. The presence of pain was found to be related with misdiagnosis (p = 0.010) (Table 3). The average time interval from symptom onset to pathological diagnosis was 3.1 months (3 days to 10 months), 2.6 months for patients with a history of misdiagnosis (10 days to 3 months) and 3.4 months (3 days to 10 months) for those without a history of misdiagnosis. The median survival time was 12 months (Table 2). The 2-year and 5-year overall survival rates were 33% and 25% respectively. The Kaplan–Meier survival analysis showed that the presence of pain (P = 0.024) and misdiagnosis (p = 0.038) were associated with a poor prognosis (Fig. 5).
RMS is a malignant mesenchymal neoplasm that exhibits striated muscle differentiation, accounting for 5–10% of all solid tumors and 55–60% of soft tissue sarcomas in the pediatric age group, but are relatively rare in adults [20, 21]. However, patients aged ≥ 20 years old accounted for 51% of this retrospective study’s sample size. The most common sites of occurrence for primary RMS were the head/neck, extremities and genitourinary tract , with the perianal and perineum area being rare sites of occurrence and considered unfavourable [1, 3, 23]. RMS in adults is more likely to occur in unfavourable sites compared to adolescents , which may explain the higher percentage of adults in this study. In addition, female predominance is noticed in this study (71%). Similar features were reported in a Japanese study , indicating regional and ethnic differences in the occurrence of this disease.
The prognosis of primary perianal and perineal RMS is extremely poor. Prognostic factors include age, pathological type, clinical group and staging . In this study, patients older than 20 years old comprised 51% of the patient population, 38% were classified as alveolar RMS and 52% were categorized into IRS groups III-IV, which may all contributed to the poorer outcomes. Besides, the rate of misdiagnosis was 45.7% in this group, which was significantly related to a poorer prognosis (p = 0.038).
PRMS cases are frequently misdiagnosed as perianal abscesses, leading to a poor prognosis. Hence, careful evaluation and differential diagnosis for suspected patients are crucial. Perianal masses are the most common manifestation of both PRMS and perianal abscesses. In fact, 97% of patients presented as perianal masses in this study. Perianal abscesses are almost always accompanied by pain . In this study, pain was found to be correlated with the probability of misdiagnosis (p = 0.010). When combined with infection, local redness, swelling and fever can be observed, which resembles the clinical presentation of perianal abscesses and thus leads to a high rate of misdiagnosis . It is difficult to distinguish PRMS from perianal abscesses solely based on symptoms. For firstly-diagnosed patients with painful perianal masses, paying attention to any delay in seeking health care, predisposing factors for abscess formation (Immune deficiency, HIV infection, diabetic ketoacidosis and Crohn's disease) and the increased levels of lactate dehydrogenase would be helpful to establish differential diagnoses . Additionally, epidemiological characteristics can provide clues suggesting that perianal abscesses mainly affect adults and male infants younger than 1 year old [28,29,30], which is different from the demographic features of PRMS reported in relevant literatures [25, 31, 32]. Empirically, whenever a young woman presents with a perianal mass, especially those under 20 years old, a differential diagnosis of solid or even malignant tumors, including PRMS should be considered. Theoretically, misdiagnosis would delay correct assessment of this disease . However, correlation between misdiagnosis and the average time interval from symptom onset to pathological diagnosis was found statistically insignificant in this study (p = 0.712). On the contrary, the average pathological diagnosis time of misdiagnosed cases was shorter than that of cases without misdiagnosis (2.6 months vs. 3.4 months). A possible reason for this finding may be the fact that patients’ complaints about pain prompted clinicians to carry out emergency abscess incision/resection, rendering an earlier acquirement of postoperative pathological results than those without pain.
Doppler ultrasound is a noninvasive, accessible and radiation-free method for preliminary examination of patients with perianal painful masses suspected to be PRMS. It has unique advantages in distinguishing solid, cystic or lacunar masses, providing high specificity and sensitivity for the diagnosis of perianal abscess. Endoanal ultrasound can clarify tumor involvement of the anal canal, and thus provide clues for accurate diagnosis and adequate treatment . Five cases of PRMS treated at our center revealed solid masses with uneven echo and rich intratumural blood flow signals with or without clear boundaries, which was consistent with the relevant reports [34,35,36]. One case indicated a perianal abscess by Doppler ultrasound, but the pathological report following a concomitant ultrasound-guided biopsy corrected the diagnosis, which could be implemented as a reliable method to further lower the risk of misdiagnosis. Endoscopic ultrasound-guided fine needle aspiration is recommended for its accuracy and minimal invasion when conducting biopsies .
The sonographic features of RMS are variable and nonspecific and have certain limitation on the assessment of deep-tissue lymph nodes and distant metastases . Hence justifying the necessity of CT or MRI for further evaluation. MRI provides clearer soft tissue imaging compared to CT scans and can better reveal the degree of invasion of the RMS in the adjacent pelvic organs. Thus, MRI has become the imaging modality of choice for the diagnosis and evaluation of pelvic RMS, but only a few reports are available for MRI description of PRMS [39,40,41]. By observing the sagittal, coronal, and axial T2 weighted high-resolution images of the pelvis, we found that the hyposignal of the external anal sphincter (EAS) is replaced by the tumour’s hypersignal in PRMS, causing a discontinuity of the anal sphincter complex. In some cases, the muscle signal of the EAS can be observed cutting into the tumour; a pseudocapsule-like structure was formed by the compressed EAS surrounding the tumour (Fig. 1). According to these features, we suggest that PRMS probably originates from the EAS. Compression rather than direct invasion of the rectal wall and anal canal was observed in 4 cases, which is different from the characteristics of anal canal cancer and thus a valuable clue for differential diagnosis [42, 43].
Among the 15 patients treated at our center, 5 underwent a previous abscess incision or drainage previously at other hospitals and 4 of them had a poor prognosis. For the 2 patients who survived for more than 5 years, one was initially diagnosed as a perianal abscess but only treated with antibiotics without surgery; both of them underwent a radical operation (R0) later on at our center and no evidence of recurrence was found after a 5-year follow up. We noticed that both the presence of pain and a history of misdiagnosis were closely related to poor outcomes. Patient’s complaining of pain usually mislead the clinician’s judgement and impelled him/her to perform the wrong surgical intervention, which may damage the integrity of the tumour and increase the difficulty of radical surgery, thus resulting in an unfavourable prognosis. Nonetheless, as pain itself may indicate local inflammation and progressive infiltration of the malignancy, such signs and symptoms could be considered as risk factors for poor outcomes independent from misdiagnosis. The relationship between pain, misdiagnosis and overall survival requires further verification. Nevertheless, if PRMS are diagnosed on time and distant metastases are excluded, surgical treatment should be carried out as soon as possible. Taking into account the possibility that this tumour likely originates from the EAS, an ELAPE (extra-levator abdominalperineal excision) could be the surgical approach of choice for most patients to achieve a R0 excision. If the tumor was relatively confined and only a small portion of the EAS was involved, a LRR with preserved anus could be performed. As a matter of fact, 5 patients received a LRR at our center and 3 of them survived for more than 5 years; 1 experienced a recurrence due to failure to achieve a R0 resection and is currently undergoing further treatment; 1 has no evidence of recurrence after 2 years of follow-up but the disease course was prolonged due to a rectovaginal fistula caused by surgical damage of the perineum. LRR can achieve long-term survival without compromising the anal function, but a R0 resection must be guaranteed and the perineum should be protected during the operation to avoid complications such as rectovaginal/rectourethral fistulae.
Optimal treatment for PRMS is controversial. Recently, a report from the CWS trials emphasized the role of surgery in the multidisciplinary management of PRMS, with its average 5y-OS reaching 47% . While another study, with a reported average 39% 5y-OS, suggested that patients undergoing surgery in addition to CRT had similar outcomes as those who received only CRT . In this group, the average 5y-OS of patients who underwent radical resection with adjuvant therapy was only 33%, which is lower than those reported by the aforementioned studies. This could probably be due to the lower implementation rate of irradiation. While the North American studies recommended RT in all RMS patients except for those in Clinical Group I ERMS, utilization of RT was more cautious in European trials due to concerns regarding its long-term damaging effects . Without a specific international guideline for RT indication in PRMS, such a measure was only applied in patients who underwent LRR to ensure better local control in our study. In fact, 4 patients who followed this treatment protocol all achieved satisfactory outcomes during our follow-ups, indicating the potential of RT in terms of local control and sphincter preservation . Inguinal lymph node metastasis is one of the main factors of poor outcomes in these patients. In this group, 11 cases were complicated with lymph node metastasis. The average 5-year OS was only 26% for the 9 patients with preoperative inguinal lymph nodes involvement. One patient had ilial lymph node metastasis within 5 months and died in 11 months following surgery. Regarding the high prevalence of regional lymph node involvement in PRMS, it is suggested that inguinal lymph nodes resection or irradiation should be performed prophylactically to control postoperative regional recurrence [26, 31], but such an aggressive approach could be avoided with the application of PET-CT scans which can effectively improve the detection rate of lymph node metastases [45, 46]. In this study, breast metastases were found in 2 cases in this study, which was also reported in a Japanese literature , providing insights on the specific metastatic pathway and histological characteristics of PRMS.
This study is mostly limited by its retrospective design, small sample size and the incomplete details from the cases included from Chinese literatures. Given these limitations, an international, large scale, multi-center study should be conducted to validate these data.
In summary, primary PRMS are rare and easily misdiagnosed soft tissue lesions, which often leads to poor outcomes. Patients presenting with painful perianal masses should be carefully evaluated to exclude this malignancy. PRMS are found to be closely related to the external anal sphincter, and MRI should be conducted to determine EAS invasion. Comprehensive therapy including radical operation, CRT and chemotherapy is often necessary and recommended. It is imperative to achieve further improvements in the clinical outcome of PRMS patients by developing new therapeutic modalities.
Availability of data and materials
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
Magnetic resonance imaging
Intergroup Rhabdomyosarcoma Study
Color Doppler flow imaging
External anal sphincter
Local radical tumor resection
Mesna + adriamycin + isocyclic amide + dacarbazine
Cyclophosphamide + vincristine + adriamycin + dacarbazine
Carboplatin + epirubicin + vincristine + dactinomycin + ifosfamide + etoposide
Fluorouracil + oxaliplatin + calcium folinate
Cisplatin + paclitaxel
Raney RB, Donaldson MH, Sutow WW, Lindberg RD, Tefft M. Special considerations related to primary site in rhabdomyosarcoma: experience of the Intergroup Rhabdomyosarcoma Study, 1972–76. Natl Cancer Inst Monogr. 1981;56:69–74.
Hill DA, Dehner LP, Gow KW, Pappo AS, Crawford D, Pflaumer SM, Furman WL, Hayes-Jordan AA, McDermott MB. Perianal rhabdomyosarcoma presenting as a perirectal abscess: a report of 11 cases. J Pediatr Surg. 2002;37:576–81.
Raney RB, Maurer HM, Anderson JR, Andrassy RJ, Donaldson SS, Qualman SJ, Wharam MD, Wiener ES, Crist WM. The Intergroup Rhabdomyosarcoma Study Group (IRSG): major lessons from the IRS-I through IRS-IV studies as background for the current IRS-V treatment protocols. Sarcoma. 2001;5:9–15.
Liu H, He HB, Wu J, Yang CM. A case of adult perianal rhabdomyosarcoma with abscess (titled in Chinese). Med J West Chin. 2011;23:2431.
Liu ZS, Yang FJ, Li D. Perianal rhabdomyosarcoma misdiagnosed as perianal abscess: analysis of two cases (titled in Chinese). Contemporary Med. 2009;0:115.
Li LQ, Liu YJ. A case of perianal rhabdomyosarcoma (titled in Chinese). Railway Med. 1993;2009(1):41.
Mo B, Xu B. A case report of perianal rhabdomyosarcoma (titled in Chinese). J Colorectal Anal Surg. 2015;021:387.
Wang JH, Yu L, Yan WX, Jia XJ. A case of perianal rhabdomyosarcoma treated by synchronous radical chemoradiotherapy (titled in Chinese). Chin J Lab Diagn. 2018;022:1590–1.
Chen YQ, Qian HH, Huang JC, Li GN. Perianal rhabdomyosarcoma: report of 3 cases and clinical analysis (titled in Chinese). Chin J Crit Care Med (Electronic Version). 2008;1:39–40.
He LZ, Wang ZQ, Yin M. Perianal rhabdomyosarcoma misdiagnosed as perianal abscess: a case report (titled in Chinese). Chin J Coloproctol. 2011;031:63.
Nie JL, Zhang YJ, Liu WY, Chen L. Perianal embryonal rhabdomyosarcoma: a case report (titled in Chinese). Chin J Lab Diagn. 2016;2016:681–2.
Liu HB, Song M, Wang LN, Liu JP, Ren XY. Primary perianal solid alveolar rhabdomyosarcoma: a case report (titled in Chinese). Guangdong Med J. 2016;037:3172.
Zong JY, Yu YD, Ji XG, Ni DB. Rhabdomyosarcoma misdiagnosed as perianal abscess: a case report (titled in Chinese). In: The 12th academic conference of colorectal diseases of Chinese Association of traditional Chinese Medicine. 2006;2.
Zhao W, Chen HM. A case of girl perianal rhabdomyosarcoma (titled in Chinese). Chin Clin Oncol. 2013;2013:100–1.
Zhu MX, Zhu N. Experience of misdiagnosis of perianal rhabdomyosarcoma: a case report (titled in Chinese). J New Med. 2007;2007:676.
Jin ML, Jiang J. A case of embryonal rhabdomyosarcoma in children (titled in Chinese). Chin J Bases Clin Gen Surg. 2005;2005:9.
Wang Y, Zou YR, Li JH. A case of perianal pleomorphic rhabdomyosarcoma in children (titled in Chinese). Sichuan J Cancer Control. 2009;2000:29.
Yu L, Zhang PD, Zhao LZ. Primary perianal embryonal rhabdomyosarcoma: a report of 2 cases (titled in Chinese). Chin J Clin Oncol. 2003;2000:3.
Jian NG, Zhou YY. Perianal alveolar rhabdomyosarcoma: a case report (titled in Chinese). Chin J Coloproctol. 2001;21:28.
Ferrari A, Dileo P, Casanova M, Bertulli R, Meazza C, Gandola L, Navarria P, Collini P, Gronchi A, Olmi P. Rhabdomyosarcoma in adults : a retrospective analysis of 171 patients treated at a single institution. Cancer-Am Cancer Soc. 2003;98:571–80.
Pappo SA. Rhabdomyosarcoma and other soft tissue sarcomas of childhood. Curr Opin Oncol. 1994;6:397–402.
Crist W, Gehan EA, Ragab AH, Dickman PS, Donaldson SS, Fryer C, Hammond D, Hays DM, Herrmann J, Heyn R, Et A. The third intergroup rhabdomyosarcoma study. J Clin Oncol. 1995;13:610–30.
Lawrence WJ, Anderson JR, Gehan EA, Maurer H. Pretreatment TNM staging of childhood rhabdomyosarcoma: a report of the Intergroup Rhabdomyosarcoma Study Group. Children’s Cancer Study Group. Pediatric Oncology Group. Cancer Am Cancer Soc. 1997;80:1165–70.
Sultan I, Qaddoumi I, Yaser S, Rodriguez-Galindo C, Ferrari A. Comparing adult and pediatric rhabdomyosarcoma in the surveillance, epidemiology and end results program, 1973 to 2005: an analysis of 2,600 patients. J Clin Oncol. 2009;27:3391–7.
Okamura K, Yamamoto H, Ishimaru Y, Takayasu H, Otani Y, Yamagishi J, Takahashi A, Kuwano H, Nagashima K, Ikeda H. Clinical characteristics and surgical treatment of perianal and perineal rhabdomyosarcoma: analysis of Japanese patients and comparison with IRSG reports. Pediatr Surg Int. 2006;22:129–34.
Casey DL, Wexler LH, LaQuaglia MP, Meyers PA, Wolden SL. Patterns of failure for rhabdomyosarcoma of the perineal and perianal region. Int J Radiat Oncol Biol Phys. 2014;89:82–7.
Marcus RH, Stine RJ, Cohen MA. Perirectal abscess. Ann Emerg Med. 1995;25:597–603.
Christison-Lagay ER, Hall JF, Wales PW, Bailey K, Terluk A, Goldstein AM, Ein SH, Masiakos PT. Nonoperative management of perianal abscess in infants is associated with decreased risk for fistula formation. Pediatrics. 2007;120:e548–52.
Serour F, Somekh E, Gorenstein A. Perianal abscess and fistula-in-ano in infants: a different entity? Dis Colon Rectum. 2005;48:359–64.
Macdonald A, Storey DW, Munro F. Treatment of perianal abscess and fistula-in-ano in children. Brit J Surg. 2003;90:221.
Blakely ML, Andrassy RJ, Raney RB, Anderson JR, Wiener ES, Rodeberg DA, Paidas CN, Lobe TE, Crist WM. Prognostic factors and surgical treatment guidelines for children with rhabdomyosarcoma of the perineum or anus: a report of intergroup rhabdomyosarcoma studies I through IV, 1972 through 1997. J Pediatr Surg. 2003;38:353.
Fuchs J, Dantonello TM, Blumenstock G, Kosztyla D, Klingebiel T, Leuschner I, Schuck A, Niggli FK, Koscielniak E, Seitz G. Treatment and outcome of patients suffering from perineal/perianal rhabdomyosarcoma: results from the CWS trials–retrospective clinical study. Ann Surg. 2014;259:1166–72.
Youssef AT. Use of ultrasonography in clarifying the etiology of anal pain. J Med Ultrasound. 2017;25:208–14.
Mak CW, Chou CK, Su CC, Huan SK, Chang JM. Ultrasound diagnosis of paratesticular rhabdomyosarcoma. Brit J Radiol. 2004;77:250–2.
Annam A, Munden MM, Mehollin-Ray AR, Schady D, Browne LP. Extratesticular masses in children: taking ultrasound beyond paratesticular rhabdomyosarcoma. Pediatr Radiol. 2015;45:1382–91.
Bahnson RR, Zaontz MR, Maizels M, Shkolnik AA, Firlit CF. Ultrasonography and diagnosis of pediatric genitourinary rhabdomyosarcoma. Urology. 1989;33:64–8.
Linea C, Sinagra E, Gioia F, Rimi C. Perianal embryonal rhabdomyosarcoma diagnosed by endoscopic ultrasound-guided fine needle aspiration. Endoscopy. 2012;4(Suppl 2 UCTN):E342–3.
Agrons GA, Wagner BJ, Lonergan GJ, Dickey GE, Kaufman MS. From the archives of the AFIP. Genitourinary rhabdomyosarcoma in children: radiologic-pathologic correlation. Radiographics. 1997;17:919–37.
Finelli A, Babyn P, Lorie GA, Bagli D, Khoury AE, Merguerian PA. The use of magnetic resonance imaging in the diagnosis and followup of pediatric pelvic rhabdomyosarcoma. J Urol. 2000;163:1952–3.
Fletcher BD, Kaste SC. Magnetic resonance imaging for diagnosis and follow-up of genitourinary, pelvic, and perineal rhabdomyosarcoma. Urol Radiol. 1992;14:263–72.
Tappouni RF, Sarwani NI, Tice JG, Chamarthi S. Imaging of unusual perineal masses. AJR Am J Roentgenol. 2011;196:W412–20.
Swami VG, Joseph K, Severin D, Tankel K, Usmani N, Nijjar T. Benefit of MRI scanning in the pretreatment assessment of anal canal carcinoma. Pract Radiat Oncol. 2013;3:S9–10.
Roach SC, Hulse PA, Moulding FJ, Wilson R, Carrington BM. Magnetic resonance imaging of anal cancer. Clin Radiol. 2005;60:1119.
Dasgupta R, Fuchs J, Rodeberg D. Rhabdomyosarcoma. Semin Pediatr Surg. 2016;25:276–83.
Ricard F, Cimarelli S, Deshayes E, Mognetti T, Thiesse P, Giammarile F. Additional benefit of F-18 FDG PET/CT in the staging and follow-up of pediatric rhabdomyosarcoma. Clin Nucl Med. 2011;36:672–7.
Volker T, Denecke T, Steffen I, Misch D, Schonberger S, Plotkin M, Ruf J, Furth C, Stover B, Hautzel H. Positron emission tomography for staging of pediatric sarcoma patients: results of a prospective multicenter trial. J Clin Oncol. 2007;25:5435–41.
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Guo, Y., Hu, B., Huang, D. et al. Perianal and perineal rhabdomyosarcomas: a retrospective multicenter study of 35 cases. BMC Surg 21, 66 (2021). https://doi.org/10.1186/s12893-021-01073-x