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Bone metastasis of malignant thymomas associated with peripheral T-cell lymphocytosis
© The Author(s). 2016
Received: 15 May 2016
Accepted: 5 August 2016
Published: 19 August 2016
Malignant thymomas are rarely associated with bone metastasis and T-cell lymphocytosis.
A 47–year-old female patient was admitted to our hospital for ptosis. A diagnosis of malignant thymoma was made based on the thymectomy and pathological result. Peripheral T-cell lymphocytosis and bone metastasis were found later. T-cell lymphocytosis was relived after surgical and radiation therapy to the metastasis.
Peripheral T-cell lymphocytosis is a rare paraneoplastic phenomenon associated with thymomas. This report is the first to describe an invasive thymoma with late bone metastasis accompanied with T-cell lymphocytosis. We should be aware of peripheral T-cell lymphocytosis in thymomas and it may contribute to a better understanding of the complex physiology and pathogenesis of thymoma.
Thymomas are rare anterior mediastinal tumors, which originate from the thymic epithelial cells. According to the pathologic classification developed by the World Health Organization, thymomas are classified into five groups (type A, AB, B1, B2 and B3) by predominate cell type. Thymomas are usually slow growing with more local recurrence rather than metastasis. Sporadic cases with late distant metastasis, including lungs, pleura, liver, thyroid and bones, have been reported .
The thymus gland is responsible for the maturation of T-lymphocyte and some other immunologic function. Thus, the neoplastic thymoma is associated with an array of “paraneoplastic” syndromes such as myasthenia gravis, pure cell aplasia, hypogammagloulinemia. However, T-cell lymphocytosis in peripheral blood is an extremely rare phenomeon.
We report a 47-year-old female patient with a previous history of myasthenia gravis (MG) and malignant thymoma, who developed thoracic vertebrae and paravertebrae metastasis associated T-cell lymphocytosis.
A 47–year-old female patient was admitted to our hospital in 2007 for ptosis. Laboratory tests showed higher percentage of the lymphocytes (77.5 %) with 4.45 × 109/L leukocytes and 3.45 × 109/L lymphocytes. A chest computed tomography scan showed an 11 cm × 9 cm × 9 cm irregular solid mass in the anterior mediastinum, with infiltration of the diaphragm and left chest wall. She was diagnosed with MG and a mediastinal mass. Thymectomy detected a mediastinal mass infiltrating the pericardium and left pleura and penetrating the left lung. In addition, second mass was found adherent to the left diaphragm. Both tumors were resected and histologically classified as malignant thymoma (type AB) with infiltration of chest wall, diaphragm, pericardium, left pleura and lung. Regional nodules were resected and showed lymphadenitis. The patient received ADOC chemotherapy (Cyclophosphamide, Doxorubicin, Cisplatin, Vincristine) and radiation therapy after surgery and followed up with a relief of MG.
The patient followed up with chest CT every year and local recurrence in the left pleura was found in April, 2010. Unfortunately, the patient was found to have peripheral T-cell lymphocytosis in 2014. Peripheral blood analysis showed mild lymphocytosis (5.3 × 109/L), with Lymphocytes representing 60.7 % of the peripheral blood leukocytes. Flow cytometric analysis revealed that most of the lymphocytes were mature CD3+ CD5 + CD7+ T cells. Moreover, bone marrow aspiration and biopsy showed normal cellular counts and percentage.
Thymomas are one of the most common tumors in the anterior mediastinum. Malignant thymomas are usually determined by the invasiveness into nearby tissues or distant metastasis. The recurrence of thymomas is rare, and distant metastasis is reported by only a few cases . In the present study, the patient was diagnosed with malignant thymoma with diaphragm and pleural infiltration at the onset. Interestingly the pathology classified the thymoma into type AB from the onset but progressed into type B2/B3 in the bone metastasis 8 years later. We hypothesized that the biological behavior of thymomas might have changed or progressed during time.
Invasive or metastasis
Pedraza, 1977 
15,000 leukocytes with 70 % lymphocytes
Griffin et al., 1978 
25,000 leukocytes with 52 % lymphocytes
Shachor et al., 1988 
28,000 leukocytes with 87 % lymphocytes
Medeiros et al., 1993 
20,000 leukocytes with 80 % lymphocytes
Smith et al., 1994 
Soft tissue of the neck/ left brachiocephalic vein
11,700 leukocytes with 59 % lymphocytes
Smith et al., 1994 
16,300 leukocytes with 52 % lymphocytes
Barton, 1997 
20,100 leukocytes with 57 % lymphocytes
Otton SH et al.2000 
Steroids & azathioprine
Morales et al., 2007 
Lung/right pleura/chest wall
13,800 leukocytes with 67 % lymphocytes
Chen HK et al., 2009 
18,000 leukocytes with 77.4 % lymphocytes
Puljiz Z et al., 2013 
34,400 leukocytes with 57 % lymphocytes
In our case, the percentage of lymphocyte was higher than normal in 2007, and T-cell lymphocytosis was diagnosed in 2013. No therapy was conducted until the patient developed lower limb weakness. Interestingly, T-cell lymphocytosis got relived immediately after the removal of the vertebral tumor. However the lymphocyte count elevated 4 days later. The radiation therapy was done and the lymphocyte counted decreased to normal again. Therefore, we concluded that T-cell lymphocytosis was associated with malignant thymoma in this case. Previous cases showed that most T-cell lymphocytosis was relived after tumor resection or chemotherapy/irradiation, indicating that lymphocytosis might be one of the paraneoplastic phenomena of thymoma. In addition, T-cell lymphocytosis may be a prediction whether the thymoma is sensitive to the therapy.
However, the mechanism has not been fully elucidated. In most cases T-cell lymphocytosis quickly resolved after surgical resection and/or chemo/radiotherapy. Earlier studies indicated a spillover from the lymphocyte-rich thymoma into the peripheral blood . However, later studies found differences between the immunophenotype of the cells in thymoma and the peripheral T-cells. Given that the immunophenotypes of circulating lymphocytes were polyclonal mature T cells, these cells were postulated to derive from the spillage of intratumoral lymphocytes into the circulation or a reactive proliferation of peripheral lymphocytes, which was related to deregulated, thymoma-associated immunoresponse. Medeiros suggested there was an immunoregulatory disorder mediated by thymic hormone released by the tumor. Most authors now agree that there may be a physiologic imbalances with malignant thymic cells, which lead to immune disorders and finally to impaired proliferation, maturation, and migration of T-cells .
Peripheral T-cell lymphocytosis is a rare paraneoplastic phenomenon associated with thymomas. We propose that female sex, lymphocyte-rich thymomas that behaved aggressively and invasively may be the risk factors in the majority of cases. As far as we know, our report is the first to describe an invasive thymoma with late bone metastasis accompanied with T-cell lymphocytosis. Clinicians should be aware of peripheral T-cell lymphocytosis in thymomas, although it is a rare manifestation of thymomas. It contributes to a better understanding of the complex physiology and pathogenesis of thymoma. Moreover, further studies are needed to investigate the relationship between T-cell lymphocytosis and thymoma.
ADOC, cyclophosphamide, doxorubicin, cisplatin, vincristine; MG, myasthenia gravis; MRI, magnetic resonance imaging
The authors appreciate the patient’s consent to present this case.
Availability of data and materials
The datasets supporting the conclusions of this article are included within the article and its additional files.
LZ, XZ, ZL and YL participated in the surgery of this case. LZ and ZL drafted the manuscript and all authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent form is available for review by the editor of this journal.
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