Gallbladder carcinoma is a rare, high malignancy neoplasm, with an incidence rate of 0,3-1,5%[1–3]. The incidence of intra or post-operative incidental gallbladder carcinoma diagnosis is estimated between 0,2 and 2,8%; in this group, 15-30% of patients prove to be asymptomatic at the time presentation, without clinical evidences, intra or pre-operative, of neoplasm [1–3]. Primary aim of our study is to evaluate incidental gallbladder carcinoma's incidence in our experience, and to correlate it with age variable; second end-point of the study is to consider the possible relation between laparoscopic cholecystectomy indication increase in our centre and neoplasm observation incidence.

For stadiation we used the Sixth Edition of AJCC TNM Manual. All laparoscopic cholecystectomies were executed with standard 4 trocars thecnique, and Endo-Bag protected gallbladder extraction.

In the matter of surgical radicalization, we executed 4b-5 segmentectomies (we did not perform right hepatectomies) [6, 7]. We always performed hepatic peduncle lymphadenectomy: if peduncle's nodes resulted positive, we executed N1 lymphadenectomy with hepatic porta and over-duodenal nodes removal. If second station nodes were suspected, we executed additional lymphadenectomy.

Resection completeness was classified in: R0 without residuals on hepatic margins, R1 microscopically positive margin, R2 macroscopic residuals on hepatic margins.

We analyzed 508 consecutive laparoscopic cholecystectomies: 457 executed for cholelytiasis (150 urgencies), 51 patients for gallbladder adenomyomathosis (all elective).

We observed 7 accidental gallbladder adenocarcinomas in post-operative histological examination (N Group - Neoplasm).

4 patients out of 7 were females (Table 1), and the mean age of Group N was 67,8 years old (range 64-75). The totality of the sample resulted in Caucasic ethnic group.

Three cholecystectomies were performed in emergency (2 for acute lythiasic cholecystitis with US evidence, and 1 for alythiasic cholecistitis in adenomyomathosis) and the other 4 in elective regime (1 for adenomyomathosis, 3 for cholelytiasis) (Table 1). Patients who underwent urgent surgery presented acute inflammatory haematic setting (Table 1). Hepato-biliary settings at admittance were normal in all patients. Intra-operative gallbladder wall morphology appeared normal in all cases. Cholecistectomies were all carried out by laparoscopy without laparotomic conversions. Gallbladder was always extracted with protections, and we didn't observe cutaneous trocar-site metastases.

Pathological results were:1 pT1b N0 (G1), 2 pT2 N0 (G2), 2 pT2 N1 (G3b), 2 pT3 N1 (G3b) (Table 1). In 5 cases we performed neoplasm radicalization surgery with standard procedure revision: hepatic 4b-5 segmentectomy, hepatic peduncle lymphadenectomy and trocar-site excisions. We didn't perform radicalization right hepatectomies. We executed a N1 lymphadenectomy, and the second station resulted intra-operatively negative. Two patients died before radicalization (both patients with pT3N1 histological finding). We didn't observe any intra-operative biliary ducts invasion, and we didn't perform any biliary resection.

Pathological results on hepatic margin were: 3 R0 and 2 R1 (these two both on pT2N1 histological result and both patients died in 1 month). 4 patients underwent adjuvant chemotherapy (Table 1).

Median global survival was 34 months; 3 patients are still alive and, at now, disease free.

In literature gallbladder cancer incidence is 0,3-1,5%; it also seems to be an increase over last years. In our study incidental gallbladder adenocarcinoma's rate resulted 1,38%. Some authors affirm that major risk factors for the disease are: female gender, obesity, age over 60 years old, and cholelythisasis [3, 4, 10–14]. According to literature, incidence resulted higher in female gender (4/7 patients), in patients with a long story of cholelythiasis (5/7), and the whole sample resulted aged over 60 (7/7). Symptoms are aspecific and the most important prognostic factor is pathological stage; also our patients resulted asymptomatic for the questioned disease [3, 4, 10–14].

Literature reported median survival rate varies between 8,1 and 68 months (range 3-100 months); in our study median survival was 34 months (range 2-63 months).

Surgical resection with curative intent in post laparoscopic cholecystectomy gallbladder carcinoma is: for stage T1a surgery is not proved to be necessary but watchful follow-up only seems to be required, for stage T1b the correct approach is still debated and some authors define as sufficient hepatic gallbladder bed resection with hepatic peduncle lymphadenectomy, for stage T2-3-4 surgical approach with hepatic S4b-5 radicalization (or more extended, "a la dèmande") is recommended, with hepatic peduncle lymphadenectomy associated to trocar-site excisions [4, 5, 8, 11, 12].

Some authors from Sloan-Kattering Institute, basing on their experience and cases, drew up a flow-chart for a correct re-evaluation method in incidentally diagnosed gallbladder cancer, suggesting always to perform an explorative laparoscopy at the beginning of radicalization surgery (that must be carried out by open technique), to exclude peritoneal carcinosis; in this way there is the possibility to better identify patients eligible for laparotomic radicalization [8].