Fig. 4

ICG detection in the linear small bowel model. Indocyanine green dye (0.1–0.3 mg/kg) was administered and the progressively de-vascularized small bowel ischemia gradient model was observed under ICG grayscale and ICG contrast mode at 30–40 s after injection. A shows ICG grayscale image of the small bowel gradient. B shows ICG contrast image of the small bowel gradient. Based on qualitative ICG images alone, a continuous gradient of bowel ischemia cannot be identified. Rather regions can be demarcated as perfused (green) 3–10 cm from stapled edge, not perfused (not green) 0–1 cm from stapled edge and suboptimal perfusion (lighter green) 1–3 cm from stapled edge