Fig. 2

Polymicrobial tibia infection confirmed by histopathological debrided sequestrum, and dermatophytosis confirmed by biopsy. The sequestrum (A–D) and surrounding inflammatory tissue (H) were collected during operation debridement, and Gram staining was performed (A) to show Gram-Positive staining in soft tissue (Yellow arrowheads) (A, B), and sequestrum bone (white and red arrowheads) (A, C, D), but adjacent skin shown chronic inflammatory cells and neutrophil infiltration (H) in 2012. The fellow-up (E–G) fungal culture-positive (I) from left heel skin biopsy in 2018