Fig. 4

Hematoxylin-eosin staining and HBME-1 immunostaining of the hepatectomy site 7 days post-surgery in an animal without adhesions. a In the control group, necrosis was observed in the hepatectomy site (asterisk). b Higher magnifications of the box in (a). A fibrous layer was formed on the resection surface (arrow). c Continuous layer of HBME-1-positive cells (black triangles) covered the fibrous layer. d In the AdSpray group, necrosis was visible in the hepatectomy site (asterisk). e Higher magnifications of the box in (d). A fibrous layer formed on the resection surface (arrow). f Continuous layer of HBME-1-positive cells (black triangles) covered the fibrous layer. g In the Seprafilm group, necrosis was visible in the hepatectomy resected site (asterisks). h Higher magnifications of the box in (g). In the Seprafilm group, the marked presence of foam cells was visible on the slit-like spaces. i Absence of HBME-1-positive cells on the resection surface in the Seprafilm group. Bars A, D, and G = 1 mm. Bars B, C, E, F, H, and I = 100 μm