Figure 4

Expression and localization of collagen type III and I. Immunofluorescence detection of immature collagen III in the control linea alba at 3 weeks (A) and 6 months (F) and in the midline closure performed using the different sutures at 3 weeks (B-E) and 6 months (G-J). Collagen fibers appear in red and cell nuclei in blue (DAPI stain). Confocal light microscopy (200x). Immunofluorescence labeling of mature collagen type I along the control linea alba at 3 weeks (K) and 6 months (P) and after closing the laparotomy incisions using the different suture threads at 3 weeks (L-O) and 6 months (Q-T). Collagen fibers appear in red and cell nuclei in blue (DAPI stain). Confocal light microscopy (200x). s: suture, LA: linea alba, PP: Surgipro™, PUe: Assuplus®, PDX: Assufil® and PDXb: Filbloc®.