Figure 4

Ethanol does not significantly affect the Ca²⁺ response to ATP. A, 20 min pre-incubation with octanol (4 mM) does not remarkably affect either the amplitude or the kinetics of the Ca²⁺ response to ATP (20 μM). The trace is the average of 16 cells from the same microscopic field of view. B, mean±SE of the peak of ATP-induced elevation in [Ca²⁺]_i. The asterisk indicates a level of significant difference <0.05. The number of ECs analyzed in each condition is indicated above the bars. C, B, mean±SE of the rate of decay of ATP-induced Ca²⁺ signal. The decay was measured by fitting the Ca²⁺ tracings with a first order exponential equation (OriginPro 7, OriginLab Corp, Northampton MA). The asterisk indicates a level of significant difference <0.05. The number of ECs analyzed in each condition is indicated above the bars.