Figure 2
NO synthesis induced by mechanical injury rat aortic endothelium. A, Ca2+ signal elicited by lesioning rat aorta in PSS. The trace, recorded from a single cell, is representative of 10 cells from the same visual field. B, injury induced an elevation in intracellular NO levels that was abolished when the cells were pre-treated with the eNOS inhibitor, L-NAME (5 mM), for 40 min. The traces are the mean±SE, respectively, of 10 (PSS, closed circles) and 5 (L-NAME, open circles) cells from two aortic rings harvested from the same animal on the same day. C, mean±SE of the NO signal recorded at increasing distance from the lesion site. In this and the following figures, the arrow indicates when injury is performed. Asterisk indicates a level of significant difference <0.05.