Fluid removal measured over a period of 10 minutes during NPWT at-50 mmHg. Measurements were performed with conventional NPWT and NPWT with a protective disc between the intestines and the vacuum source. The results are shown as means ± the SEM of six experiments. Statistical analysis was performed using the Mann-Whitney test. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) and p > 0.05 (not significant, n.s.).