Microscopy of alkylene oxide-treated defects at 17 days post-surgery. Alkylene oxide copolymer-treated adjacent 5 μm demineralized bone sections stained with H&E (upper) and Trichrome-EVG at ×2 (left) and ×10 magnification. The edge of the defect is identified with black/green arrows, and the area of magnification shown with a dotted box. Defects show good healing with newly formed woven bone (light blue arrows) rich in cellular activity (osteoid shown with dark blue arrows). Blue stars indicate native collagen meshwork throughout the tissue.